Journal article
J. J. Sajkowska-Kozielewicz, P. Kozielewicz, K. Makarova, Marcin Stocki, N. Barnes, K. Paradowska
Molecules, 2020
Molecules, 2020
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DOI
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APA
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Sajkowska-Kozielewicz, J. J., Kozielewicz, P., Makarova, K., Stocki, M., Barnes, N., & Paradowska, K. (2020). Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark. Molecules.
Chicago/Turabian
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Sajkowska-Kozielewicz, J. J., P. Kozielewicz, K. Makarova, Marcin Stocki, N. Barnes, and K. Paradowska. “Geissospermiculatine, a New Alkaloid from Geissospermum Reticulatum Bark.” Molecules (2020).
MLA
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Sajkowska-Kozielewicz, J. J., et al. “Geissospermiculatine, a New Alkaloid from Geissospermum Reticulatum Bark.” Molecules, 2020.
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@article{j2020a,
title = {Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark},
year = {2020},
journal = {Molecules},
author = {Sajkowska-Kozielewicz, J. J. and Kozielewicz, P. and Makarova, K. and Stocki, Marcin and Barnes, N. and Paradowska, K.}
}
Abstract
A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, 1H-15N HMBC). Cytotoxic properties were evaluated in vitro on malignant THP-1 cells, and the results demonstrated that the cytotoxicity of the alkaloid (30 μg/mL) was comparable with staurosporine (10 μM). Additionally, the toxicity was tested on zebrafish (Danio rerio) embryos in vivo by monitoring their development (0–72 h); toxicity was not evident at 30 μg/mL.